Macrogen
MACROGEN Customized Sequencing without gDNA extraction *
Inclusive of Standard PCR amplification of a target region using Macrogen's default PCR conditions.
Inclusive of PCR clean-up and bidirectional sequencing.
Results released within 2-3 working days. Inclusive of free shipping.
Inclusive of fundamental analysis (investigation of homology for protein. DNA sequence level through BlastN or BlastX). With complimentary MOPC-purified universal primers for sequencing. Free one-time resequencing for inconclusive results. DNA Sequencing Facility: Macrogen Inc., Korea
1.00
MACROGEN Customized Sequencing with gDNA extraction *
Inclusive of gDNA extraction of Microorganisms (bacteria and fungi only). Samples must be pure isolates on agar plate or glycerol stock.
Standard PCR amplification of a target region using Macrogen's default PCR conditions.
Inclusive of PCR clean-up and bidirectional sequencing.
Results released within 2-3 working days. Inclusive of free shipping.
Inclusive of fundamental analysis (investigation of homology for protein. DNA sequence level through BlastN or BlastX). With complimentary MOPC-purified universal primers for sequencing. Free one-time resequencing for inconclusive results. DNA Sequencing Facility: Macrogen Inc., Korea
1.00
MACROGEN PCR Purification.
1.00
MACROGEN PCR Purification
1.00
MACROGEN Gel Extraction
Applicable for PCR products with multiple bands. The target band is extracted prior to sequencing. Clients must provide the target band size information for the sample.
1.00
MACROGEN Standard PCR amplification
1.00
MACROGEN Customized PCR amplification
1.00
MACROGEN Gradient PCR
1.00
MACROGEN gDNA Extraction for Microorganisms
Samples must be pure isolates on agar plate or glycerol stock.
1.00
MACROGEN gDNA Extraction - Animal, Insect, Tissue
1.00
MACROGEN Difficult Sequencing
Applicable for templates with High GC or GT, unusual secondary structure, repetitive sequence, and siRNA with hairpin structure.
1.00
MACROGEN Species Identification (16s, 18s, ITS, 26s)
Inclusive of DNA extraction, PCR amplification, bidirectional sequencing, and contig assembly.
For 16S: PCR on 16S rRNA using 27F and 1492R primers; and yielding of 1,300bp or more sequencing data using internal primers 785F, 907R.
For ITS: primers to be used are ITS5 and ITS4 with >500bp guaranteed length. Inclusive of bioinformatics report.
For 18s: primers to be used are NS1 and NS24.
For 26s (D1,D2,D3 region): primers to be used are LR0R and LR7.
Turnaround time: 4-5 days upon arrival of samples at Macrogen.
1.00
MACROGEN Fragment Analysis (GeneScan) service
1.00
MACROGEN Fragment Analysis (GeneScan) service
1.00
MACROGEN Fragment Analysis (GeneScan) service
1.00
MACROGEN Fragment Analysis (GeneScan) service
1.00
MACROGEN Primer Walking
Reads 2~15kbp of sequence with PCR product or plasmid DNA that cannot be read fully by single primer extension sequencing.
1.00
MACROGEN Cloning
Inclusive of:
Cloning x 1 sample
Plasmid preparation x 3 colonies
Standard sequencing single x 6 reactions (3 samples, bidirectional)
1.00
MACROGEN Cell Line Authentication
Service product: AmpFISTR Identifiler PCR amplification KIT(16STR marker).
Sample type: Genomic DNA (100ng/ul).
Turnaround time (TAT): 3-5 business days upon arrival of samples at Macrogen Korea.
Inclusive of free shipping
1.00
MACROGEN Cell Line Authentication
Service product: APowerplex 21 system KIT(21 STR marker).
Sample type: Genomic DNA (100ng/ul).
Turnaround time (TAT): 3-5 business days upon arrival of samples at Macrogen Korea.
Inclusive of free shipping
1.00
MACROGEN Cell Line Authentication
Service product: AmpFISTR Identifiler PCR amplification KIT(16STR marker).
Sample type: Cell pellet (~10,000 cells -visible cell pellet).
Turnaround time (TAT): 3-5 business days upon arrival of samples at Macrogen Korea
Inclusive of free shipping
1.00
MACROGEN Cell Line Authentication
Service product: APowerplex 21 system KIT(21 STR marker).
Sample type: Cell pellet (~10,000 cells -visible cell pellet).
Turnaround time (TAT): 3-5 business days upon arrival of samples at Macrogen Korea
Inclusive of free shipping
1.00
MACROGEN Gene Synthesis Service.
1.00
MACROGEN Infinium Asian Screening Array (ASA)
1.00
Illumina Infinium Rice 7K Array
1.00
MACROGEN Axiom Genome Wide Chicken Genotyping Array
1.00
MACROGEN Custom Oligo/Primer Synthesis.
MACROGEN Custom Oligo/Primer Synthesis.
Delivery w/in 3-8 working days.
Scale:. 25 nmole, 50nmole, 200nmole, 1000nmole
Oligos are delivered as lyophilized product.
1.00
MACROGEN Duplex Oligo Annealing Service - 0.05 umole
1.00
MACROGEN Duplex Oligo Annealing Service - 0.2 umole
1.00
MACROGEN Duplex Oligo Annealing Service - 1 umole
1.00
MACROGEN Pre-Designed CRISPR/Cas9 Plasmid Synthesis (1~2ug)
This is a plasmid synthesis service based on a pre-designed gRNA for the desired gene. The plasmid is all-in-one format with "U6-sgRNA + CAG-Cas9" structure. Package inclusions: CRISPR plasmid (1~2ug). Plasmid type (selection marker): EGFP, PuroR. Turnaround time: 2 weeks. Note: Client must provide the gRNA sequence (20 bps) to order. If own gRNA sequence is not available, please use the Custom-Designed service.
1.00
MACROGEN Pre-Designed CRISPR/Cas9 sgRNA Synthesis (20ug)
This is a sgRNA synthesis service based on pre-designed gRNA for the desired gene. Package inclusions: CRISPR sgRNA (20ug) + Nuclease free water. Cas9 protein is not included. Turnaround time: 2 weeks. Note: Client must provide the gRNA sequence (20 bps) to order. If own gRNA sequence is not available, please use the Custom-Designed service.
1.00
MACROGEN Pre-Designed CRISPR/Cas9 sgRNA Synthesis (50ug)
This is a sgRNA synthesis service based on pre-designed gRNA for the desired gene. Package inclusions: CRISPR sgRNA (50ug) + Nuclease free water. Cas9 protein is not included. Turnaround time: 2 weeks. Note: Client must provide the gRNA sequence (20 bps) to order. If own gRNA sequence is not available, please use the Custom-Designed service.
1.00
MACROGEN Pre-Designed CRISPR/Cas9 RNP (Ribonucleoprotein) package
This is a plasmid synthesis service based on a pre-designed gRNA for the desired gene and is supplied with Cas9 protein. Package inclusions: CRISPR sgRNA (50ug) + Cas9 protein (50ug) + Nuclease free water. Turnaround time: 2 weeks. Note: Client must provide the gRNA sequence (20 bps) to order. If own gRNA sequence is not available, please use the Custom-Designed service.
1.00
MACROGEN Custom-Designed CRISPR/Cas9 Plasmid Synthesis (1~2ug)
This is a plasmid synthesis service based on a custom-designed gRNA for the desired gene. The plasmid is all-in-one format with "U6-sgRNA + CAG-Cas9" structure. Package inclusions: CRISPR plasmid (1~2ug). Plasmid type (selection marker): EGFP, PuroR. Turnaround time: 2 weeks. Note: Free gRNA design service is included. However, the activity of the gRNA is not guaranteed. Macrogen only considers the off-target issue and gene structure (exons, introns and isoforms) in designing the gRNA.
1.00
MACROGEN Custom-Designed CRISPR/Cas9 sgRNA Synthesis (20ug)
This is a plasmid synthesis service based on a custom-designed gRNA for the desired gene. Package inclusions: CRISPR sgRNA (20ug) + Nuclease free water. Cas9 protein is not included. Turnaround time: 2 weeks. Note: Free gRNA design service is included. However, the activity of the gRNA is not guaranteed. Macrogen only considers the off-target issue and gene structure (exons, introns and isoforms) in designing the gRNA.
1.00
MACROGEN Custom-Designed CRISPR/Cas9 sgRNA Synthesis (50ug)
This is a plasmid synthesis service based on a custom-designed gRNA for the desired gene. Package inclusions: CRISPR sgRNA (50ug) + Nuclease free water. Cas9 protein is not included. Turnaround time: 2 weeks. Note: Free gRNA design service is included. However, the activity of the gRNA is not guaranteed. Macrogen only considers the off-target issue and gene structure (exons, introns and isoforms) in designing the gRNA.
1.00
MACROGEN Custom-Designed CRISPR/Cas9 RNP (Ribonucleoprotein) package
This is a plasmid synthesis service based on a custom-designed gRNA for the desired gene and is supplied with Cas9 protein. Package inclusions: CRISPR sgRNA (50ug) + Cas9 protein (50ug) + Nuclease free water. Turnaround time: 2 weeks. Note: Free gRNA design service is included. However, the activity of the gRNA is not guaranteed. Macrogen only considers the off-target issue and gene structure (exons, introns and isoforms) in designing the gRNA.
1.00
MACROGEN Cas9 protein 50 ug
This service provides Cas9 (tagged with NLS) protein (from S.pyogenes). Package inclusions: Cas9 protein (50 ug) + Nuclease free water. Turnaround time: 2 weeks.
1.00
MACROGEN Cas9 protein 200 ug
This service provides Cas9 (tagged with NLS) protein (from S.pyogenes). Package inclusions: Cas9 protein (200 ug) + Nuclease free water. Turnaround time: 2 weeks.
1.00
MACROGEN Cas9 protein 500 ug
This service provides Cas9 (tagged with NLS) protein (from S.pyogenes). Package inclusions: Cas9 protein (500 ug) + Nuclease free water. Turnaround time: 2 weeks.
1.00
MACROGEN Cas9 protein 1000 ug
This service provides Cas9 (tagged with NLS) protein (from S.pyogenes). Package inclusions: Cas9 protein (1000 ug) + Nuclease free water. Turnaround time: 2 weeks.
1.00
MACROGEN CRISPR/Cas9 Plasmid Validation
This is a plasmid synthesis service based on custom-designed gRNA for the desired gene with guaranteed activity. The activity is measured by in-vitro cleavage assay. The plasmid is all-in-one format with "U6-sgRNA + CAG-Cas9" structure. Package inclusions: <= 4 CRISPR plasmids (1~2ug). Plasmid type (selection marker): EGFP or PuroR. Turnaround time: 8 weeks.
1.00
MACROGEN CRISPR/Cas9 sgRNA Validation
This is a a sgRNA synthesis service based on custom-designed gRNA for the desired gene with guaranteed activity. The activity is measured by in-vitro cleavage assay. The plasmid is all-in-one format with "U6-sgRNA + CAG-Cas9" structure. Package inclusions: <= 4 CRISPR sgRNAs (50ug) + Cas9 protein (50ug) + Nuclease free water. Turnaround time: 8 weeks.
1.00